#: locale=en ## Hotspot ### Text HotspotPanoramaOverlayTextImage_6866C4E6_75B5_22B1_419E_7F71CFF6F5A9.text = Back to Tissue Processor HotspotPanoramaOverlayTextImage_6F5DCC10_7595_E190_41D5_558A53F91E82.text = Click above to continue lab tour HotspotPanoramaOverlayTextImage_6262335E_749F_6791_41D5_AA1C7331234A.text = Embedding HotspotPanoramaOverlayTextImage_6A53094A_75B5_23F0_41CB_8A3C71D7E3B2.text = Microscope HotspotPanoramaOverlayTextImage_7BB2A89D_7494_E290_41DC_16BE93B79F8D.text = Microtome HotspotPanoramaOverlayTextImage_6283A925_7477_63B0_41C5_B1DF69331080.text = Staining HotspotPanoramaOverlayTextImage_6D95A547_759D_E3F0_41D8_564E2FC63461.text = Tissue Processor ### Tooltip HotspotPanoramaOverlayArea_6CF10579_7493_6390_41DB_8E2A8C827628.toolTip = Click here for video HotspotPanoramaOverlayArea_7A41F701_7497_6F73_4196_CD4258CAA1B0.toolTip = Click here for video HotspotPanoramaOverlayArea_6D8905E5_759F_62B0_41DA_1701E3F4FB9D.toolTip = Click here for video HotspotPanoramaOverlayArea_62213F67_7475_3FB0_41CD_7BA2B5350AB5.toolTip = Click here for video HotspotPanoramaOverlayArea_6902F90A_75B7_6370_4189_EC9804581E1A.toolTip = Click here for video ## Media ### Audio audiores_6E7900CF_75BD_228F_41AD_6A67F8164F0F.mp3Url = media/audio_6C6B08E8_75BC_E2B0_41CF_BDD733F95009_en.mp3 ### Image imlevel_6F866C98_75BD_E290_41C4_09AA41994249.url = media/panorama_7E12C370_74F5_2790_41D9_0E410C322DF1_HS_4aeiqvka_en.png imlevel_6FCEFD21_75BD_E3B0_41D1_03F9D3EE4599.url = media/panorama_7E12C370_74F5_2790_41D9_0E410C322DF1_HS_r3b9offn_en.png imlevel_63AD0A77_758F_E190_41C2_C94B0302499B.url = media/panorama_7E1F4D8F_74F5_2370_41C4_561CF8FAEE24_HS_9ph5jg7o_en.png imlevel_628C6414_7493_6190_4196_F37E480FAF35.url = media/panorama_7E1F4D8F_74F5_2370_41C4_561CF8FAEE24_HS_bhncyel0_en.png imlevel_628E03E4_7493_66B1_41D8_258AB965A6D5.url = media/panorama_7E1F4D8F_74F5_2370_41C4_561CF8FAEE24_HS_fc78d9t2_en.png imlevel_6B80992E_758C_E3B1_41C1_20F85A7FB728.url = media/panorama_7E1F4D8F_74F5_2370_41C4_561CF8FAEE24_HS_x8zd58l1_en.png imlevel_6B86195B_758C_E390_41AD_345338462FA0.url = media/panorama_7E1F4D8F_74F5_2370_41C4_561CF8FAEE24_HS_zkuub4zb_en.png ### Title panorama_7E12C370_74F5_2790_41D9_0E410C322DF1.label = Histology Entry panorama_7E1F4D8F_74F5_2370_41C4_561CF8FAEE24.label = Histology Lab video_79502F1C_7493_1F90_41CA_9E5C1E850866.label = RP 190515 Histology 00 video_78E4F2F5_7493_2690_41B0_32EA96358BB9.label = RP 190515 Histology 01 video_78E5463B_7493_2190_41B5_643EE085E2A5.label = RP 190515 Histology 02 video_78E4C9BE_7493_2290_41D0_B2A3FE80923E.label = RP 190515 Histology 03 video_78E4FD33_7493_2390_41D9_5F411964E698.label = RP 190515 Histology 04 album_6FA38ED3_75B3_1E97_4189_A381C9BA29BA_0.label = Tissue Processor album_6FA38ED3_75B3_1E97_4189_A381C9BA29BA.label = Tissue Processor ### Video videolevel_529A738F_758D_E770_41D2_7BD31DDF3B3A.url = media/video_78E4C9BE_7493_2290_41D0_B2A3FE80923E_en.mp4 videolevel_529A738F_758D_E770_41D2_7BD31DDF3B3A.posterURL = media/video_78E4C9BE_7493_2290_41D0_B2A3FE80923E_poster_en.jpg videolevel_5298D3CB_758D_E6F7_41C9_54672AD9B11E.url = media/video_78E4F2F5_7493_2690_41B0_32EA96358BB9_en.mp4 videolevel_5298D3CB_758D_E6F7_41C9_54672AD9B11E.posterURL = media/video_78E4F2F5_7493_2690_41B0_32EA96358BB9_poster_en.jpg videolevel_529A836F_758D_E7B0_4180_B3F0FF03793F.url = media/video_78E4FD33_7493_2390_41D9_5F411964E698_en.mp4 videolevel_529A836F_758D_E7B0_4180_B3F0FF03793F.posterURL = media/video_78E4FD33_7493_2390_41D9_5F411964E698_poster_en.jpg videolevel_529BF3A9_758D_E6B0_41D8_C13135A72E41.url = media/video_78E5463B_7493_2190_41B5_643EE085E2A5_en.mp4 videolevel_529BF3A9_758D_E6B0_41D8_C13135A72E41.posterURL = media/video_78E5463B_7493_2190_41B5_643EE085E2A5_poster_en.jpg videolevel_529993EA_758D_E6B1_41C8_3386EEF984CC.url = media/video_79502F1C_7493_1F90_41CA_9E5C1E850866_en.mp4 videolevel_529993EA_758D_E6B1_41C8_3386EEF984CC.posterURL = media/video_79502F1C_7493_1F90_41CA_9E5C1E850866_poster_en.jpg ## Skin ### Multiline Text HTMLText_607F982D_7495_61B0_41D4_9B02C6CEF37B.html =
Embedding


The specimen is thoroughly infiltrated with wax and it must be formed into a “block” to allow it to be clamped into a microtome for sections to be cut.


This step is carried out using an “embedding centre” where a mould is filled with molten wax and the specimen placed into it.
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Microscope


The tissue samples can now be viewed under the microscope.
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Microtome


Once the tissues have been embedded, they must be cut into very thin sections that can be placed on a slide. This is done with a microtome. The cut sections are placed on a water bath.


The preferred sections are picked up using a glass slide. The sections are then heat fixed. It is not ready for the next step, staining.
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Staining


The embedding process must be reversed in order to get the paraffin wax out of the tissue and allow water soluble dyes to penetrate the sections. Therefore, before any staining can be done, the slides are "deparaffinized" by running them through xylenes (or substitutes) to alcohols to water. There are no stains that can be done on tissues containing paraffin.


The staining process makes use of a variety of dyes that have been chosen for their ability to stain various cellular components of tissue. The routine stain is that of hematoxylin and eosin (H and E). All other stains are referred to as "special stains" and are done.
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Tissue Processor


The technique of getting fixed tissue into paraffin is called tissue processing. The main steps in this process are dehydration and clearing. Fixed tissue samples cannot be directly infiltrated with paraffin.


First, the water from the tissues must be removed by dehydration. This is usually done with a series of alcohols, such as 70% to 95% to 100%.


The next step is called "clearing" and consists of removal of the dehydrant with a substance that will be miscible with the embedding medium (paraffin). The commonest clearing agent is xylene.


Finally, the tissue is infiltrated with the embedding agent (paraffin). The above processes are automated in a histology laboratory using a tissue processor , which is an instrument that moves the tissues around through the various agents on a preset time scale.
## Tour ### Description ### Title tour.name = Histology Lab