#: locale=en

## Hotspot

### Text
HotspotPanoramaOverlayTextImage_6866C4E6_75B5_22B1_419E_7F71CFF6F5A9.text = Back to Tissue Processor
HotspotPanoramaOverlayTextImage_6F5DCC10_7595_E190_41D5_558A53F91E82.text = Click above to continue lab tour
HotspotPanoramaOverlayTextImage_6262335E_749F_6791_41D5_AA1C7331234A.text = Embedding
HotspotPanoramaOverlayTextImage_6A53094A_75B5_23F0_41CB_8A3C71D7E3B2.text = Microscope
HotspotPanoramaOverlayTextImage_7BB2A89D_7494_E290_41DC_16BE93B79F8D.text = Microtome
HotspotPanoramaOverlayTextImage_6283A925_7477_63B0_41C5_B1DF69331080.text = Staining
HotspotPanoramaOverlayTextImage_6D95A547_759D_E3F0_41D8_564E2FC63461.text = Tissue Processor

### Tooltip
HotspotPanoramaOverlayArea_6CF10579_7493_6390_41DB_8E2A8C827628.toolTip = Click here for video
HotspotPanoramaOverlayArea_7A41F701_7497_6F73_4196_CD4258CAA1B0.toolTip = Click here for video
HotspotPanoramaOverlayArea_6D8905E5_759F_62B0_41DA_1701E3F4FB9D.toolTip = Click here for video
HotspotPanoramaOverlayArea_62213F67_7475_3FB0_41CD_7BA2B5350AB5.toolTip = Click here for video
HotspotPanoramaOverlayArea_6902F90A_75B7_6370_4189_EC9804581E1A.toolTip = Click here for video

## Media

### Audio
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### Image
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### Title
panorama_7E12C370_74F5_2790_41D9_0E410C322DF1.label = Histology Entry
panorama_7E1F4D8F_74F5_2370_41C4_561CF8FAEE24.label = Histology Lab
video_79502F1C_7493_1F90_41CA_9E5C1E850866.label = RP 190515 Histology 00
video_78E4F2F5_7493_2690_41B0_32EA96358BB9.label = RP 190515 Histology 01
video_78E5463B_7493_2190_41B5_643EE085E2A5.label = RP 190515 Histology 02
video_78E4C9BE_7493_2290_41D0_B2A3FE80923E.label = RP 190515 Histology 03
video_78E4FD33_7493_2390_41D9_5F411964E698.label = RP 190515 Histology 04
album_6FA38ED3_75B3_1E97_4189_A381C9BA29BA_0.label = Tissue Processor
album_6FA38ED3_75B3_1E97_4189_A381C9BA29BA.label = Tissue Processor

### Video
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## Skin

### Multiline Text
HTMLText_607F982D_7495_61B0_41D4_9B02C6CEF37B.html = <div style="text-align:left; color:#000; "><DIV STYLE="text-align:left;font-size:37px;"><SPAN STYLE="display:inline-block; letter-spacing:0px; white-space:pre-wrap;color:#000000;font-family:Arial, Helvetica, sans-serif;"><SPAN STYLE="font-size:37px;"><B>Embedding</B></SPAN></SPAN></DIV><p STYLE="margin:0; line-height:22px;"><BR STYLE="display:inline-block; letter-spacing:0px; white-space:pre-wrap;color:#000000;font-size:12px;font-family:Arial, Helvetica, sans-serif;"/></p><DIV STYLE="text-align:left;font-size:22px;"><SPAN STYLE="display:inline-block; letter-spacing:0px; white-space:pre-wrap;color:#000000;font-family:Arial, Helvetica, sans-serif;"><SPAN STYLE="font-size:22px;"><B>The specimen is thoroughly infiltrated with wax and it must be formed into a “block” to allow it to be clamped into a microtome for sections to be cut. </B></SPAN></SPAN></DIV><p STYLE="margin:0; line-height:22px;"><BR STYLE="display:inline-block; letter-spacing:0px; white-space:pre-wrap;color:#000000;font-size:12px;font-family:Arial, Helvetica, sans-serif;"/></p><DIV STYLE="text-align:left;font-size:22px;"><SPAN STYLE="display:inline-block; letter-spacing:0px; white-space:pre-wrap;color:#000000;font-family:Arial, Helvetica, sans-serif;"><SPAN STYLE="font-size:22px;"><B>This step is carried out using an “embedding centre” where a mould is filled with molten wax and the specimen placed into it.</B></SPAN></SPAN></DIV></div>
HTMLText_62E86419_7473_6190_41C7_D3737D515CBB.html = <div style="text-align:left; color:#000; "><DIV STYLE="text-align:left;font-size:37px;"><SPAN STYLE="display:inline-block; letter-spacing:0px; white-space:pre-wrap;color:#000000;font-family:Arial, Helvetica, sans-serif;"><SPAN STYLE="font-size:37px;"><B>Microscope</B></SPAN></SPAN></DIV><p STYLE="margin:0; line-height:22px;"><BR STYLE="display:inline-block; letter-spacing:0px; white-space:pre-wrap;color:#000000;font-size:12px;font-family:Arial, Helvetica, sans-serif;"/></p><DIV STYLE="text-align:left;font-size:22px;"><SPAN STYLE="display:inline-block; letter-spacing:0px; white-space:pre-wrap;color:#000000;font-family:Arial, Helvetica, sans-serif;"><SPAN STYLE="font-size:22px;"><B>The tissue samples can now be viewed under the microscope.</B></SPAN></SPAN></DIV></div>
HTMLText_616CE371_748F_2790_41D6_4AC39B9A4371.html = <div style="text-align:left; color:#000; "><DIV STYLE="text-align:left;font-size:37px;"><SPAN STYLE="display:inline-block; letter-spacing:0px; white-space:pre-wrap;color:#000000;font-family:Arial, Helvetica, sans-serif;"><SPAN STYLE="font-size:37px;"><B>Microtome</B></SPAN></SPAN></DIV><p STYLE="margin:0; line-height:22px;"><BR STYLE="display:inline-block; letter-spacing:0px; white-space:pre-wrap;color:#000000;font-size:12px;font-family:Arial, Helvetica, sans-serif;"/></p><DIV STYLE="text-align:left;font-size:22px;"><SPAN STYLE="display:inline-block; letter-spacing:0px; white-space:pre-wrap;color:#000000;font-family:Arial, Helvetica, sans-serif;"><SPAN STYLE="font-size:22px;"><B>Once the tissues have been embedded, they must be cut into very thin sections that can be placed on a slide. This is done with a microtome.  The cut sections are placed on a water bath. </B></SPAN></SPAN></DIV><p STYLE="margin:0; line-height:22px;"><BR STYLE="display:inline-block; letter-spacing:0px; white-space:pre-wrap;color:#000000;font-size:12px;font-family:Arial, Helvetica, sans-serif;"/></p><DIV STYLE="text-align:left;font-size:22px;"><SPAN STYLE="display:inline-block; letter-spacing:0px; white-space:pre-wrap;color:#000000;font-family:Arial, Helvetica, sans-serif;"><SPAN STYLE="font-size:22px;"><B>The preferred sections are picked up using a glass slide. The sections are then heat fixed. It is not ready for the next step, staining.</B></SPAN></SPAN></DIV></div>
HTMLText_62277E48_7473_21F0_41AD_E6C662A48A80.html = <div style="text-align:left; color:#000; "><DIV STYLE="text-align:left;font-size:37px;"><SPAN STYLE="display:inline-block; letter-spacing:0px; white-space:pre-wrap;color:#000000;font-family:Arial, Helvetica, sans-serif;"><SPAN STYLE="font-size:37px;"><B>Staining</B></SPAN></SPAN></DIV><p STYLE="margin:0; line-height:22px;"><BR STYLE="display:inline-block; letter-spacing:0px; white-space:pre-wrap;color:#000000;font-size:12px;font-family:Arial, Helvetica, sans-serif;"/></p><DIV STYLE="text-align:left;font-size:22px;"><SPAN STYLE="display:inline-block; letter-spacing:0px; white-space:pre-wrap;color:#000000;font-family:Arial, Helvetica, sans-serif;"><SPAN STYLE="font-size:22px;"><B>The embedding process must be reversed in order to get the paraffin wax out of the tissue and allow water soluble dyes to penetrate the sections. Therefore, before any staining can be done, the slides are "deparaffinized" by running them through xylenes (or substitutes) to alcohols to water. There are no stains that can be done on tissues containing paraffin. </B></SPAN></SPAN></DIV><p STYLE="margin:0; line-height:22px;"><BR STYLE="display:inline-block; letter-spacing:0px; white-space:pre-wrap;color:#000000;font-size:12px;font-family:Arial, Helvetica, sans-serif;"/></p><DIV STYLE="text-align:left;font-size:22px;"><SPAN STYLE="display:inline-block; letter-spacing:0px; white-space:pre-wrap;color:#000000;font-family:Arial, Helvetica, sans-serif;"><SPAN STYLE="font-size:22px;"><B>The staining process makes use of a variety of dyes that have been chosen for their ability to stain various cellular components of tissue. The routine stain is that of hematoxylin and eosin (H and E). All other stains are referred to as "special stains" and are done.</B></SPAN></SPAN></DIV></div>
HTMLText_6C437EAF_758F_1EB0_41BE_36C19203EAFB.html = <div style="text-align:left; color:#000; "><DIV STYLE="text-align:left;font-size:37px;"><SPAN STYLE="display:inline-block; letter-spacing:0px; white-space:pre-wrap;color:#000000;font-family:Arial, Helvetica, sans-serif;"><SPAN STYLE="font-size:37px;"><B>Tissue Processor</B></SPAN></SPAN></DIV><p STYLE="margin:0; line-height:22px;"><BR STYLE="display:inline-block; letter-spacing:0px; white-space:pre-wrap;color:#000000;font-size:12px;font-family:Arial, Helvetica, sans-serif;"/></p><DIV STYLE="text-align:left;font-size:24px;"><SPAN STYLE="display:inline-block; letter-spacing:0px; white-space:pre-wrap;color:#000000;font-family:Arial, Helvetica, sans-serif;"><SPAN STYLE="font-size:24px;"><B>The technique of getting fixed tissue into paraffin is called tissue processing. The main steps in this process are dehydration and clearing. Fixed tissue samples cannot be directly infiltrated with paraffin. </B></SPAN></SPAN></DIV><p STYLE="margin:0; line-height:24px;"><BR STYLE="display:inline-block; letter-spacing:0px; white-space:pre-wrap;color:#000000;font-size:12px;font-family:Arial, Helvetica, sans-serif;"/></p><DIV STYLE="text-align:left;font-size:24px;"><SPAN STYLE="display:inline-block; letter-spacing:0px; white-space:pre-wrap;color:#000000;font-family:Arial, Helvetica, sans-serif;"><SPAN STYLE="font-size:24px;"><B>First, the water from the tissues must be removed by dehydration. This is usually done with a series of alcohols, such as 70% to 95% to 100%. </B></SPAN></SPAN></DIV><p STYLE="margin:0; line-height:24px;"><BR STYLE="display:inline-block; letter-spacing:0px; white-space:pre-wrap;color:#000000;font-size:12px;font-family:Arial, Helvetica, sans-serif;"/></p><DIV STYLE="text-align:left;font-size:24px;"><SPAN STYLE="display:inline-block; letter-spacing:0px; white-space:pre-wrap;color:#000000;font-family:Arial, Helvetica, sans-serif;"><SPAN STYLE="font-size:24px;"><B>The next step is called "clearing" and consists of removal of the dehydrant with a substance that will be miscible with the embedding medium (paraffin). The commonest clearing agent is xylene.  </B></SPAN></SPAN></DIV><p STYLE="margin:0; line-height:24px;"><BR STYLE="display:inline-block; letter-spacing:0px; white-space:pre-wrap;color:#000000;font-size:12px;font-family:Arial, Helvetica, sans-serif;"/></p><DIV STYLE="text-align:left;font-size:24px;"><SPAN STYLE="display:inline-block; letter-spacing:0px; white-space:pre-wrap;color:#000000;font-family:Arial, Helvetica, sans-serif;"><SPAN STYLE="font-size:24px;"><B>Finally, the tissue is infiltrated with the embedding agent (paraffin). The above processes are automated in a histology laboratory using a tissue processor , which is an instrument that moves the tissues around through the various agents on a preset time scale.</B></SPAN></SPAN></DIV></div>

## Tour

### Description

### Title
tour.name = Histology Lab